ZFIN Image: Ju et al., 2014, Fig. S2

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Figure Caption

Fig. S2

The early and late phenotypes of the Tg(krt5:Gal4VP16;14×UAS:smoa1-EGFP) stable transgenic fish. (a) Double transgenic embryo at 30 hpf derived from crossing Tg(krt5:Gal4VP16;14×UAS:smoa1-EGFP) and Tg(UAS-E1b:nfsB-mCherry)^c264 lines. Note the GFP expression in the head region (arrow). (b) The same embryo showing mCherry expression not only in the head region, but also in the skin epithelia (arrow). (c) Dorsal view of a 96 hpf wild-type embryo. (c′) Transverse cryosection of the same embryo showing proper closure of the choroid fissure. (d) Transgenic embryo at 96 hpf showing a coloboma phenotype (arrow), transverse sectioning showing the choroid fissures failed to close (d′, arrow). (e) A two-week-old double transgenic juvenile showing ectopic cell proliferation at the ciliary marginal zones (arrows). (f) A 45-day old zebrafish showing failure to close the choroid fissure (arrow), a feature characteristic of coloboma.

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Acknowledgments

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