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Fig. 2
MeS Neuron Morphologies Revealed by Single-Cell Electroporations
(A–C) Examples of confocal image projections of the midbrain in 6-day-old Gal4s1171t/UAS:GFP fish. MeLm (A), MeS type 1 (B), and MeS type 2 (C) are shown. Cells were electroporated with tetramethylrhodamine (TMR) dextran (magenta). GFP (green) is driven by Gal4s1171t. TMR is brighter than GFP in cellular processes. The dendrites therefore appear magenta. The somas of electroporated cells are double-labeled by GFP and TMR and appear as white.
(D–F) Drawings of the nMLF cells in (A)–(C) showing their axonal projections. The corresponding photomicrographs are provided in Figure S2. Axon termination points are indicated by red arrowheads. Rostral collaterals in the hindbrain are indicated by blue arrowheads.
(G) Summary table for all electroporations performed.
See also Figure S2.