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Fig. S1 Morphological changes of nitrite-exposed embryos at early development. Nitrite-exposed embryos were treated with 100 mg/l sodium nitrite from 10 hpf. They displayed a similar morphological phenotype to control embryos at 24 hpf (A, B). At 48 hpf, the nitrite-exposed embryos (D) looked very similar to control embryos (C) except that they showed a slightly shortened body length (D). When reaching 76 hpf, the nitrite-exposed embryos displayed normal diameter of eye, and heart development (E, G) when compared to control embryos (F, G). However, the body length of the exposed embryos was significantly shorter than control embryos (F, G). At 84 hpf, some of nitrite-exposed embryos started to exhibit cardiac edema (H, I).