ZFIN Image: Zhao et al., 2014, Fig. 6

Image

Figure Caption

Fig. 6

Ribosome biogenesis defects in fan mutant zebrafish.

(A) Northern blot analysis of rRNA isolated from 30 and 50 hpf wild type (W) and fan mutants (M) using an oligonucleotide probe against the 52ETS region of zebrafish pre-rRNA. Pre-rRNAs (a) and (b) are indicated. Methylene blue (MB) staining of the mature 28S and 18S rRNAs was carried out as a loading control. Quantitation of the ration of a/b was performed using Image J. (B) Western blot of whole-cell extracts treated with WDR43 or EGFP control siRNA as indicated. (C) qRT-PCR analyses of human WDR43 gene expression level in non-treated (NT), GFP or WDR43 siRNA treated HeLa cells normalized to β -actin. (D, E) Subcellular localization of mCherry-tagged Utp15 (red) in control GFP (D) and WDR43 (E) siRNA treated HeLa cells counter stained with DAPI (blue).

Figure Data

Phenotype details

Acknowledgments

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