ZFIN Image: Zhao et al., 2014, Fig. 2

Image

Figure Caption

Fig. 2

fan encodes zebrafish Wdr43/Utp5.

(A) Left side: Chromosomal position of the fan locus (left). Sequencing trace data of wild type and fan mutant alleles, with red arrow indicating a premature stop codon in fan mutants (right). Comparison of the conserved nature of the amino acid mutated in fan zebrafish (purple) (middle). Schematic of the Wdr43 protein domains and fan mutant predicted premature stop codon (bottom). (B–E) Live images of zebrafish treated with control MO (CMO) or fan MO at indicated developmental times. (F–I) Alcian blue stained wild type (F, H) and fan mutant (G, I) embryos injected at the single cell stage with GFP control (ctr) or wild type wdr43 mRNA. (I) Injected wdr43 mRNA rescued fan mutant cartilage formation. (J) Percentage of control and wdr43 mRNA injected fan mutant clutches exhibiting wild type, intermediate and fan mutant pharyngeal arch cartilage formation. Numbers of injected embryos scored are indicated at the top of each bar.

Figure Data

Phenotype details

Acknowledgments

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