ZFIN Image: Gao et al., 2014, Fig. 7

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Figure Caption

Fig. 7

The myofibril defects from smyd1b knockdown could be rescued by ectopic expression of zebrafish smyd1a or mouse Smyd1.

The smyd1b ATG-MO was co-injected with smyd1b:zfsmyd1a^myc or smyd1b:mSmyd1^myc transgene into zebrafish embryos at 1 or 2 cells stages. Myosin thick filament organization and transgene expression were analyzed by double immunostaining with anti-MHC (F59, green) and anti-myc (9E10, red) antibodies. A, C, E. Double immunostaining with anti-MHC (A) and anti-myc (C) antibodies shows the normal thick filament organization in myofibers expressing the myc-tagged zebrafish smyd1a transgene at 24 hpf. E, merged picture of A and C. B, D, F. Double immunostaining with anti-MHC (B) and anti-myc (D) antibodies shows the normal thick filament organization in myofibers expressing the myc-tagged mouse Smyd1 transgene at 24 hpf. F, merged picture of B with D. D and F showed the sarcomeric localization of myc-tagged mouse Smyd1 (red). The myc-tagged mouse Smyd1 was localized in the middle of the MHC thick filament (F), a region normally occupied by the M-line. Scale bars: 20 μm in E; 14 μm in F.

Acknowledgments

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