ZFIN Image: Priyadarshini et al., 2013, Fig. 4

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Figure Caption

Fig. 4

In-situ hybridization and q-RT-PCR at 3dpf in wild type fish following treatment with H₂O₂ and H₂O₂+LGR.

A. The th2 transcript levels significantly decreased in the H₂O₂ treated samples, verified by q-RT-PCR.

B. The pink1 transcript levels increased significantly in the H₂O₂ treated as compared to wild type samples.

C. No significant change in the th1 transcript levels was detected between the groups. Only a declining trend was observed.

Unpaired t-test (* p-value < 0.05, ** p-value< 0.01).

D-F. The expression of th2 mRNA is reduced in group 10b of the 7-dpf H₂O₂-treated fish as compared with untreated control fish. In the LGR-treated groups at 8 dpf, the decrease in th2 mRNA expression was prevented. The th2-expressing neuron groups 3b, 8b, 9b, 10b are identified according to [26].

Scale bar represents 100 μM. th1 – tyrosine hydroxylase1, th2 – tyrosine hydroxylase 2, pink1 – PTEN-induced putative kinase 1.

Figure Data

Expression / Labeling details

Acknowledgments

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