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Fig. 5
Ptpn11a-/- and double mutant embryos exhibit craniofacial defects at 5 dpf.
Alcian blue staining was done to visualize the cartilage. (a,e) wild-type, (b,f) ptpn11a-/-, (c,g) ptpn11b-/- and (d,h) double homozygous mutant. (i) Average width of the ceratohyal angle (indicated in panel a) was determined for each genotype (n = 10–24). Statistics were determined using a student′s t-test; ** indicates a p value of <0.01, *** indicates a p value <0.001.
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