|
Fig. 4
Yap is directly involved in zebrafish retinogenesis.
(A) Schematic illustration of the base hsp70-EGFP-yap construct (left panel) and the procedure for the heat shock experiment (right panel). Zebrafish embryos at the one-cell stage were injected with plasmid DNA containing the heat shock promoter constructs indicated in (B). At 21 hpf, injected embryos were immersed in a 37°C water bath for 1 h to apply heat shock and thus induce expression of EGFP-fused Yap. At 54 hpf, EGFP-expressing embryos were isolated and classified on the basis of their phenotypic features. (B) Representative images of the embryos in (A) that were injected with heat shock promoter constructs as indicated on the left side of panels. For each column, top right panels show lateral views of whole embryos, top left panels show higher magnification images of the head regions of the embryos, and bottom panels are fluorescent images of the corresponding top panels. White arrowheads, areas of AP. (C) Quantification of phenotypes of the embryos injected with heat shock promoter constructs in (A, B) as analyzed at 54 hpf. Color classification is as for Fig. 1A except that the phenotype of AP alone is indicated by striped orange shading. Results are presented as the percentage of the total number of embryos examined (N).