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Fig. 8
Cyp26 enzymes act non-autonomously to promote cranial endothelial specification. (A) Transplant protocol. Tg(kdrl:mCherry) embryos were injected with a blue lineage tracer. Donor cells are then transplanted into the marginal zone of host embryos and scored for endothelial contribution at 48hpf. (B,C) Representative Tg(kdrl:mCherry) donor cells that incorporated into the cranial vasculature and trunk vasculature. (D) Transplant results indicating that wild-type donor cells incorporate significantly less frequently into cranial vasculature and endocardium in Cyp26-deficient host embryos than in wild-type host embryos. Numbers in parentheses indicate the number of embryos that had contributed to the designated tissue relative to the total embryos. Images are lateral views with anterior rightwards. Significant differences compared with controls are indicated (*P<0.05).