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Fig. S2
flt4, ccbe1 and vegfc are required for secondary sprouting from the PCV
(A-D) Confocal images of 1.5 dpf lyve1:egfp embryos treated with (A) control MO (B) flt4 MO (C) ccbe1 MO (D) vegfc MO-1. Secondary sprouts from the PCV (arrowheads) are only present in embryos injected with control MO. (E) Quantitation of secondary sprout formation at 1.5 dpf in embryos injected with morpholinos. *** p<0.001, by a Mann-Whitney test to control; error bars indicate s.d. Scale bar: 50 μm.
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