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Fig. S9
Macrophage depletion is maintained in adult caudal fins through 14 dpa. (A,B) Transverse and flat sections of Tg(mpeg1:NTR-YFP) amputated caudal fins at 7 dpa and 14 dpa with continuous MTZ treatment (NTR+MTZ) and without (NTR-MTZ). (C) Flow cytometry of pooled 14 dpa fins gated for YFP+ (macrophages) and Dapi-( live cells) in NTR-MTZ and NTR+MTZ fish. (D) Quantification of flow cytometry of Dapi-YFP+ population as a percentage of total events for 14 dpa pooled fins Error bars represent the s.e.m. data representative of 2 separate experiments. (E) Representative fin images (YFP) for temporally ablated fish at 3 dpa (where either MTZ was washed out or MTZ added for the first time) and 5 dpa. Scale bar = 200 μm.