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Fig. S4
Neutrophil deficiency near amputation plane does not affect regeneration. (A) Experimental scheme. Adult Tg(mpo:GFP) fish were incubated in diphenyleneiodonium chloride (DPI) at 30 μM for 12 h before caudal fins were amputated with a distal resection. DPI was added to fish water daily for 3 dpa. After 3 dpa, fish were incubated in water without DPI for the rest of the experiment (10 dpa). Yellow arrowheads represent cut plane. (B) Neutrophil (mpo+) density near the injury area was reduced with DPI treatment. Representative images of mpo+ cells near the amputation plane at 2 dpa. Scale bar = 100 μm. (C) Rate of fin regeneration was not significantly different between DPI-treated (DPI) (N=7) and untreated (DMSO) (N=6) fish for duration of experiment. Error bars represent the s.e.m. Data representative of two separate experiments.