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Fig. 6
Expression analysis confirms the lack of a systematic developmental delay following tmem88a knockdown. Markers specific to the endothelium (fli1), developing muscle (myoD) and hematopoiesis (beta-globin E3) were visualized using in situ hybridization at 24 (A-I), 48 (J-R) and 72 (S-AA) hpf in wild-type embryos (A-C,J-L,S-U) and following injection of either the ATG (D-F,M-O,V-X) or splice-blocking (G-I,P-R,Y-AA) tmem88a morpholinos. Whereas fli1 and myoD expression appears unaltered, beta-globin E3 shows a slight elevation in expression following tmem88a knockdown at 48 hpf, which is largely alleviated by 72 hpf. High-resolution images of any panel are available upon request.