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Fig. 4
tmem88a sensitizes the wnt11 cyclopia phenotype. Embryonic zebrafish were injected with low doses of morpholinos targeting either wnt11 or tmem88a (both ATG targeting and splice blocking) and morphants scored for cyclopia at 48 hpf. Cyclopia classes (A) are as previously defined (Marlow et al., 1998), such that in class 1 eye spacing is comparable to that of wild type, class 2 the spacing is decreased, class 3 eyes are marginally fused, class 4 eyes are completely fused, and class 5 have one eye. The proportion by class (B) is significantly different (P<0.05, Chi-squared test) for both wnt11 + tmem88a ATG and wnt11 + tmem88a splice morpholino co-injections as compared with wnt11 morpholino alone.