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Fig. 3
Ectopic Eda expression restores upper pharyngeal teeth to the zebrafish. Lower pharyngeal teeth (arrows) in lateral (A) and transverse (B) views of wild-type alizarin red-stained larvae. (C and D) bmp2b expression is limited to tooth germs (arrows) of the lower pharynx in wild-type. Upper pharyngeal teeth (arrowheads) in lateral (E) and transverse (F) views of ef1α:eda transgenic zebrafish. (G) bmp2b expression is induced in the upper pharyngeal epithelium (arrowhead) by ectopic eda expression and becomes limited to tooth germs (arrowhead) at later stages (H). Abbreviations: cb5, fifth ceratobranchial; cl, cleithrum; cp, chewing pad; d, dentary bone; n, notochord; y, yolk. (Scale bars, 50 μm.)