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Fig. S5
Quantification of intersomitic artery (ISA) and vein (ISVe) connections. (A) Surface renderings of trunk blood vessel 2-photon micrographs following angiography with QDots. Dorsal aorta (DA) and posterior cardinal vein (PCV) denoted by brackets. Connection of an intersomitic vessel to the DA (white arrow; defined as an ISA) and PCV (light blue arrowhead; defined as an ISVe) are indicated in the first panel. (B) Graph showing average number of ISA and ISV connections per 10 vessels in embryos of indicated genotype, or injected with indicated Morpholino. Intersomitic vessels were quantified as arterial or venous based on circulation at 72 hpf in at least 10 embryos for each genotype or Morpholino injection. For Morpholino injections, embryos were injected with either 2.5 ng control or 2.5 ng notch1b Morpholino.