ZFIN Image: Quillien et al., 2014, Fig. S2

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Figure Caption

Fig. S2

(A) DIC image of wild type embryo at 22 hours post fertilization (hpf) following whole mount in situ hybridization with a riboprobe against notch1a. White arrows indicate low level expression in presumptive endothelial cells in the dorsal aorta. (B) RT-PCR of a notch1b fragment spanning exon 8. The expected fragment size with normal splicing is approximately 600 bp, which is indicated on the marker lane. (C) Dorsal-lateral view of head region in a notch3^fh332 mutant embryo injected with 2.5 ng notch1b MO. White arrows denote red blood cells that have leaked out of the cranial vessels indicative of hemorrhage.

Acknowledgments

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