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Fig. S3
UHRF1 overexpression in hepatocytes induces senescence independent of apoptosis. (Related to Figure 3)
(A) TUNEL assay was used to assess apoptosis in uhrf1 mutant fish and UHRF1-GFP High fish. Apoptotic cells were seen in 5 dpf uhrf1 mutants, but not in the livers of UHRF1-GFP High fish. Images were taken at 10x. (B) Senescence associated β-galactosidase (SA-β-gal) staining in the liver was scored for each of the three UHRF1-GFP transgenic lines and control larvae on 5 dpf. The number of fish scored and the number of clutches (in parenthesis) are indicated.
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Reprinted from Cancer Cell, 25(2), Mudbhary, R., Hoshida, Y., Chernyavskaya, Y., Jacob, V., Villanueva, A., Fiel, M.I., Chen, X., Kojima, K., Thung, S., Bronson, R.T., Lachenmayer, A., Revill, K., Alsinet, C., Sachidanandam, R., Desai, A., SenBanerjee, S., Ukomadu, C., Llovet, J.M., and Sadler, K.C., UHRF1 overexpression drives DNA hypomethylation and hepatocellular carcinoma, 196-209, Copyright (2014) with permission from Elsevier. Full text @ Cancer Cell