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Fig. S4
Responses of tg+;dlc- embryos to a series of 4 standard heat-shocks (15+25 minutes), delivered (A) at the 3-somite stage and (B) at the 13-15 somite stage, with fixation at 48hpf. Within each class, all embryos (n=127 for A, n=32 for B) showed a similar distribution of rescued somites – anterior for A, about 12 somite-widths further posterior for B – as illustrated by the photographs. In each case, the rescue of segmentation begins with a delay of 3 to 5 segmentation clock cycles relative to the time of heat-shock. Asterisks mark rescued somite boundaries. Details of staining etc. as in Fig. 3. (C) Measurement of whole tg-;dlC+ and tg+dlC- (15+60 minutes treatment) embryos. (D) Graph of embryo length measurements. Note that heat shocked tg-;dlC+ (n=10) and tg+dlC- (n=11) embryos have similar body lengths.