ZFIN Image: Nissim et al., 2014, Fig. S4

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Figure Caption

Fig. S4

Liver vs. Pancreas Specification of Mouse Bipotential Endoderm Progenitors is Influenced by PGE2 (Related to Figure 4) (A) Mouse bipotential endodermal progenitors are derived from mouse embryonic stem cells by a step-wise differentiation protocol as shown. (B) Bipotential liver-pancreas endodermal progenitors are detected by triple co-staining for the hepatopancreatic markers Prox1 (green) and Onecut1 (red) as well as the pan-endoderm marker Foxa2 (blue). (C) Bipotential endodermal progenitors exposed to optimal pancreas conditions will be specified into a predominantly Pdx1+ population, whereas progenitors exposed to optimal liver conditions will be specified into a predominantly Hnf4α+ population. Scale bar = 100 µm.

Acknowledgments

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Reprinted from Developmental Cell, 28(4), Nissim, S., Sherwood, R.I., Wucherpfennig, J., Saunders, D., Harris, J.M., Esain, V., Carroll, K.J., Frechette, G.M., Kim, A.J., Hwang, K.L., Cutting, C.C., Elledge, S., North, T.E., and Goessling, W., Prostaglandin E2 regulates liver versus pancreas cell-fate decisions and endodermal outgrowth, 423-437, Copyright (2014) with permission from Elsevier. Full text @ Dev. Cell