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Fig. 5
Smad4 and Max Are Required in the YSL for BMP Signaling in the Embryo
Embryos injected into the YSL with 8 ng of maxmisMO (A–E), maxMO (F–J), smad4misMO>YSL (K–O), or smad4MO>YSL (P–T).
(A, A2, F, F2, K, K2, P, and P2) Image of a live morphants at prim-5.
(B, G, L, and Q) egr2b expression at prim-5.
(C, H, M, and R) gata1 expression at 8-somites (12 hpf).
(D, I, N, and S) pSmad1/5 gradient at 75% epiboly.
(E, J, O, and T) bmp2b/swr expression at 75% epiboly.
(U) The presence (blue) or absence (red) of the ventral tailfin was scored in smad4MO>YSL morphants injected with 100 pg β-galactosidase mRNA, 100 pg HA-smad4 mRNA, or 25 pg bmp2b/swr mRNA. Tailfin defects (red) were also scored in maxMO>YSL morphants injected with 100 pg β-galactosidase mRNA, 100 pg HA-max mRNA, or 25 pg bmp2b/swr mRNA. Anterior is to the left in (A)–(C), (F)–(H), (K)–(M), and (P)–(R). Dorsal is to the right in (D), (E), (I), (J), (N), (O), (S), and (T). p d 0.05.
See also Figures S2 and S5.
Reprinted from Developmental Cell, 28(3), Sun, Y., Tseng, W.C., Fan, X., Ball, R., and Dougan, S.T., Extraembryonic signals under the control of MGA, Max, and Smad4 are required for dorsoventral patterning, 322-334, Copyright (2014) with permission from Elsevier. Full text @ Dev. Cell