ZFIN Image: Zhao et al., 2014, Fig. S1

Image

Figure Caption

Fig. S1

Knockdown efficiency of wdr43 Morpholino. (A) Diagram of the wdr43 reporter construct used to test wdr43 MO knockdown efficiency. GFP (green box) was fused in frame to the 32 end of a portion of the 52 end of the wdr43 cDNA including the 52 UTR (purple line) and first two exons of wdr43 gene (purple box). Red line indicates the MO target region. The chimeric gene was driven by the CMV promoter and followed by the 32 SV40 polyA signal (PA). (B–B3) Fluorescence of shield stage zebrafish embryos injected with the wdr43-GFP reporter construct and control MO (CMO) (B, fluorescent microscopy; B2 bright field, B3 merged fluorescent and bright field). (C–C) Lack of fluorescence in shield stage zebrafish embryos injected with wdr43-GFP reporter constructs and wdr43 MO (C, fluorescent microscopy; C2 bright field; C3 merged fluorescent and bright field).

Acknowledgments

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