ZFIN Image: Dash et al., 2014, Fig. S2

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Figure Caption

Fig. S2

Sept7b is required for the development of photoreceptor outer segment. (A) Hematoxylin-eosin stained histological section of 4 dpf zebrafish larva showing the photoreceptor cell layer (p). The photoreceptor outer segment, that comprises modified cilia, is marked with an arrowhead. (B) Histological section of 4 dpf sept7b translation blocking antisense morpholino oligonucleotide (TBMO)-treated zebrafish larva shows shortening of the photoreceptor (p) outer segment cilia (arrowhead). (C-D) Immunofluorescence staining of 4 dpf control (C) and sept7b TBMO-treated zebrafish (D) for septin (red) and acetylated tububulin (green). (C) Septin 7 localizes in a punctate fashion in the photoreceptor cells in the control, and the photoreceptor outer segment is visualized by acetylated tubulin (arrowheads). (D) The photoreceptor outer segment cilia are absent or clearly shorter in sept7b TBMO-treated zebrafish larva. Scale bar: 25 μm (A-B); 10μm (C-D).

Acknowledgments

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