ZFIN Image: Wehner et al., 2014, Fig. 3

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Figure Caption

Fig. 3

Wnt/β-Catenin Signaling Must Be Inhibited in the Distal and Proximal Blastema for Regenerative Growth to Be Stalled

(A) Cartoon showing strategy for tissue-specific inducible overexpression of the Wnt/β-catenin signaling inhibitor axin1 using the TetON system.

(B) ubiquitin promoter-driven axin1 overexpression in the epidermis and the blastema is sufficient to block regenerative growth.

(C) keratin4 promoter-driven axin1 overexpression in the epidermis excluding the basal layer does not interfere with regenerative growth.

(D) keratin18 promoter-driven axin1 overexpression in the basal epidermal layer (and ectopic expression in the proximal medial blastema) does not interfere with regenerative growth.

(E) axin1 overexpression in the committed sp7⁺ osteoblasts does not interfere with regenerative growth.

(F) axin1 overexpression in the medial proximal blastema has only little impact on regenerative growth in her4.3:TetA AmCyan; TetRE:Axin1-YFP double transgenic fish. White arrowhead: distal-most blastema.

In (B)–(F), BF, bright field. Small arrowheads, amputation plane. Scale bars, 200 μm (whole mounts); 100 μm (sections). Error bars indicate error of the mean.

Acknowledgments

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