ZFIN Image: Perathoner et al., 2014, Fig. S4

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Figure Caption

Fig. S4

Polarization of fins during growth. Voltage sensitive dyes were used to assess changes in overall polarization of growing caudal fins of wild type and alf juvenile fish. (A) DiSBAC₂(3) staining in wild type fins exhibited hyperpolarization localized to discrete regions of the fin with variable detection of distal regions of altered depolarization. (B) alf fins in contrast show high levels of depolarization across the fin with variable patterns in different tissues. (C) Quantification of average DiSBAC₂(3) fluorescence signal in wild type and mutant fins (average pixel intensity (12-bits) of the fin in maximum intensity projections). ***: p<0.001, N = 21–23. (D) Positive control of depolarization by treatment of the fins with 150 mM KCl (D2). DiBAC₄(3), another dye sensitive to depolarization, showed similar effects, while DiSC₃(5), a dye sensitive to hyperpolarized states, was uninformative (data not shown).

Acknowledgments

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