ZFIN Image: West et al., 2014, Fig. 6

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Figure Caption

Fig. 6

Transactivation in the Tg(TRE:HA-Crb2a^IntraWT) line. (A) Diagram indicating the cross of Tg(opn1sw1:rtTA, TRE:GFP) to Tg(TRE:HA-Crb2a^IntraWT) for double-transgenic embryo production. (B–D) Confocal z-projection of a retinal section from a 6 dpf Tg(opn1sw1:rtTA, TRE:GFP; TRE:HA-Crb2a^IntraWT) larva treated with Dox for 72 h and labeled with anti-HA antibody. (B) GFP fluorescence (green) and (C) anti-HA immunofluorescence (red) indicate that HA-Crb2a^IntraWT is expressed in UV cones (D, merge, yellow). (E) Confocal z-projection of an untreated 6 dpf Tg(opn1sw1:rtTA, TRE:GFP; TRE:HA-Crb2a^IntraWT) retinal section labeled with anti-HA antibody. (F) Diagram indicating the cross of Tg(opn1sw1:rtTA^flag) to Tg(TRE:HA-Crb2a^IntraWT) for double-transgenic embryo production. (G–I) Confocal z-projection of a retinal section from a 6 dpf Tg(opn1sw1:rtTA^flag; TRE:HA-Crb2a^IntraWT) larva treated with Dox for 72 h and labeled with anti-FLAG and anti-HA antibodies. (G) Anti-FLAG immunofluorescence (green) and (H) anti-HA immunofluorescence (red) indicate HA-Crb2a^IntraWT is expressed in the UV cones (I, merge, yellow). (J) Confocal z-projection of an untreated retinal section from a 6 dpf Tg(opn1sw1:rtTA^flag; TRE:HA-Crb2a^IntraWT) larva labeled with anti-HA antibody. Scale bar (J), 10 μm.

Acknowledgments

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Reprinted from Gene expression patterns : GEP, 14(2), West, M.C., Campbell, L.J., Willoughby, J.J., and Jensen, A.M., Two types of transgenic lines for doxycycline-inducible, cell-specific gene expression in zebrafish ultraviolet cone photoreceptors, 96-104, Copyright (2014) with permission from Elsevier. Full text @ Gene Expr. Patterns