ZFIN Image: West et al., 2014, Fig. 4

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Figure Caption

Fig. 4

Generation of UV-cone-specific, epitope-tagged, rtTA-expressing transgenic line for doxycycline-inducible gene expression. (A) Diagram of the construct used to generate the stable transgenic zebrafish line, Tg(opn1sw1:rtTA^flag); alb^/, which expresses a FLAG-tagged rtTA protein specifically in UV cone photoreceptors. The UV opsin promoter (opn1sw1) drives expression of the flag-epitope tagged reverse tetracycline-controlled transcriptional activator (rtTA^flag) gene. (B, C) Confocal z-projection of a retinal section from a 6 dpf Tg(opn1sw1:rtTA^flag); alb^/ larva labeled with anti-FLAG and anti-UV opsin antibodies. (B) Anti-FLAG immunofluorescence (green) is visible in the photoreceptor layer of the retina. (C) Anti-UV opsin immunofluorescence (red) colocalizes with the anti-FLAG immunofluorescence (green) in UV opsin cone photoreceptors. dA, polyadenylation signal; Tol2, pTol integration site. Scale bar (C), 50 μm.

Acknowledgments

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Reprinted from Gene expression patterns : GEP, 14(2), West, M.C., Campbell, L.J., Willoughby, J.J., and Jensen, A.M., Two types of transgenic lines for doxycycline-inducible, cell-specific gene expression in zebrafish ultraviolet cone photoreceptors, 96-104, Copyright (2014) with permission from Elsevier. Full text @ Gene Expr. Patterns