ZFIN Image: Wang et al., 2013, Fig. S2

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Figure Caption

Fig. S2

Sequence information and morphological phenotype of new gli2a mutant alleles. (A) Sequence information of gli2a^i275/+and gli2a^i276/+at ZFN target site. (B–G) Morphological phenotype of wild type, gli2a^i276/i276, gli1^ts269/ts269, gli1^ts269/ts269;gli2a^i276/i276, gli2a^ty119/ty119, and gli2a^i276/ty119 at 30 hpf. (H) Alignment of Gli2a and Gli2aⁱ²⁷⁶ amino acid sequence. Sequences highlighted in yellow show the common amino acids between them, sequence in red indicates the antigen region for generating the Gli2a antibody used in this study. Sequences underlined indicate the Gli2a repressor domain (blue), zinc finger DNA binding domain (pink), nuclear localization sequences (black) and activator domain (green). All domains were annotated by alignment with the human GLI2 protein (Fernandez-Zapico, 2008).

Acknowledgments

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