Fig. 1
Ethanol treatment affects the development of larval zebrafish.
Zebrafish embryos were raised in varying concentrations of ethanol (0% through 1.5% by volume) from 2 days post-fertilization (dpf) to 5 dpf, fixed, and imaged. (A) Untreated control animals appear normal. (B) 1% ethanol-treated animals have a slightly swollen heart (arrow) and no swim bladder but otherwise appear normal. (C) Increasing the concentration of ethanol treatment to 1.5% resulted in embryos exhibiting swollen hearts (arrow), swollen guts (#), missing a swim bladder (white asterisk), and inhibited craniofacial development (arrowhead), which is similar to the phenotype exhibited by children with FAS.