ZFIN Image: Viringipurampeer et al., 2014, Fig. 1

Image

Figure Caption

Fig. 1

Genotyping and retinal histology for mutant pde6c^w59 zebrafish. (a) Genotyping for mutation status using BsaX1 restriction digest of pde6c PCR amplicon. Wild-type (+/+) band is 157bp and the mutant (/) band is 122bp. (b) Heterozygous (+/) and mutant pde6c phenotype on transgenic background of GFP-expressing cone photoreceptors at 4 and 7 days post fertilization (d.p.f.) showing degeneration of cones. Scale bar=20μm. (c) Comparison of sections through heterozygous (+/) and mutant (–/–) retinas at 7>d.p.f. C, degenerating cones (black arrows); gcl, ganglion cell layer; inl, inner nuclear layer; onl, outer nuclear layer; r, single layer of rods (white arrowheads); black arrowheads, preserved peripheral retina. Scale bar=50μm. Dorsal retina is at the top of each panel. (d) Higher magnification of heterozygous (het) versus mutant (mt) retina at 4d.p.f. cn, cone nuclei; ipl, inner plexiform layer; rn, rod nuclei. Scale bar=μm. White arrows: condensed rod nuclei

Figure Data

Expression / Labeling details

Phenotype details

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Cell Death Differ.