ZFIN Image: Johnson et al., 2014, Fig. 8

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Fig. 8 Kif11 is required for motorneurons and CoSA interneurons. Wild type/vehicle control (A,C,E,G) and kif11^-/-/STLC treated embryos (B,D,F,H) were labeled for anti-GABA (A,B,E,F) to label distinct DoLA, CoSA, VeLD, and KA interneurons (denoted D,C,V, and K, respectively), as well as anti-ISL (C,D,G,H) to label Rohon Beard sensory neurons and motor neurons (denoted R and M, respectively). Decreases in GABA+ cell populations as well as axon pathfinding errors were found in Kif11 deficient embryos as compared with controls (asterisks in B and F label cell body, arrowheads mark axon trajectory). (I) Quantification of neuronal populations identified decreases specifically in CoSA and MN populations within Kif11 deficient embryos (asterisks, p<0.05) as compared with controls. (J) Time course analysis of GABA+ cell types at 24 hpf, 30 hpf and 36 hpf shows a consistent number of GABA+ cells both lost and retained over time in kif11 mutants and STLC treated embryos.

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Acknowledgments

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Reprinted from Developmental Biology, 387(1), Johnson, K., Moriarty, C., Tania, N., Ortman, A., Dipietrantonio, K., Edens, B., Eisenman, J., Ok, D., Krikorian, S., Barragan, J., Golé, C., and Barresi, M.J., Kif11 dependent cell cycle progression in radial glial cells is required for proper neurogenesis in the zebrafish neural tube, 73-92, Copyright (2014) with permission from Elsevier. Full text @ Dev. Biol.