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Fig. 5
Involvement of MAPK-sox9a and p53 pathways in morphological alterations of cultured gonadal tissue. Ovary tissues were sliced and cultured in vitro. After 2–3 days, MKI or DMSO control were added and tissues were incubated for 48h. The cultures were costained with Erk1/2 and p-Erk1/2 antibodies (left two columns) or costained with sox9a and p53 antibodies (right two columns). DAPI staining and DOC were seen. Apoptotic or degenerating cells (arrow) and related signal staining (dotted circle) are shown. Scale bars=20μm
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