ZFIN Image: Ivanovitch et al., 2013, Fig. S4

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Figure Caption

Fig. S4

(A-B) wildtype and laminin-γ1 morphants, respectively, expressing H2b-RFP and Pard3- GFP. Arrows in (B) point at mis-positioned pard3-GFP puncta in the eye field of the laminin- γ1 morphant.

(C) laminin-γ1 morphant immunostained as detailed in the panel. Arrows point at mispositioned ZO-1 puncta.

(D) laminin-γ1 morphant cells (green) transplanted into a wild type eye field show no overt phenotype. Embryo immunostained as detailed in the panel.

(E-F) frontal and dorsal views, respectively, of 3ss Tg(rx3:GFP) embryos showing laminin-β1 mRNA accumulation (red) and GFP protein (green). laminin-α1 and laminin-γ1 show similar expression patterns (not shown).

(G-H) BSA-coated (G) and Laminin1-coated (H) beads implanted in the eye field. Embryos were left to develop until 10ss and immunostained as detailed in the panels.

(I-L) Frontal views of 24hpf embryos of the genotypes detailed in the panels.

Acknowledgments

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Reprinted from Developmental Cell, 27(3), Ivanovitch, K., Cavodeassi, F., and Wilson, S.W., Precocious acquisition of neuroepithelial character in the eye field underlies the onset of eye morphogenesis, 293-305, Copyright (2013) with permission from Elsevier. Full text @ Dev. Cell