ZFIN Image: Wylie et al., 2014, Fig. 2

Image

Figure Caption

Fig. 2

UTR1 and UTR2 suppress gene expression in cis. (A) Schematic diagram of reporter transgenes utilizing the wnt8a promoter. UTRs (arrows indicating directionality) are ligated into a unique ClaI restriction site. The two transcription start sites present in the promoter are indicated by bent arrows. (B, E, H, and K) Bright field and (C, F, I, and L) fluorescent images of live transgenic 24 hpf embryos, lateral views. (D, G, J, and M) Tails of transgenic embryos after in situ hybridization for EGFP transcripts. Arrows indicate positions of tail tips. (B–D) Reporters with UTR1 are expressed at a significantly lower level than reporters with the UTR1-reverse complement (E–G). (H–J) Reporters with UTR2 are expressed at a significantly lower level than reporters with the UTR2-reverse complement (K–M).

Figure Data

Expression / Labeling details

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image.

Reprinted from Developmental Biology, 386(1), Wylie, A.D., Fleming, J.A., Whitener, A.E., and Lekven, A.C., Post-transcriptional regulation of wnt8a is essential to zebrafish axis development, 53-63, Copyright (2014) with permission from Elsevier. Full text @ Dev. Biol.