ZFIN Image: Wang et al., 2014, Fig. S3

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Figure Caption

Fig. S3

Endothelial cell differentiation in notch3^fh332 mutant larvae. (A-H) Representative results of BBB assay in 4 dpf wildtype and mutant larvae using 10KD FITC-dextran and Evans blue dye. Insets, higher magnification view of brain microvessels. (AD) Mutant embryos failed to retain 10KD FITC-dextran in the brain microvessels 20 min after the injection. (E-H) Mutant embryos failed to retain Evans blue dye in the brain microvessels 30 min after the injection. (I-L) ISH showing cdh5 and cldn5b expression in the brain of 3 dpf wild-type and notch3^fh332 larvae. Arrowheads, endothelial expression of cdh5 or cldn5b. (M-P) Electron microscopy images of brain microvessels in 4 dpf wild-type and notch3^fh332 mutant larvae. RB, red blood cell. E, endothelial cell. Arrowheads, endothelial tight junctions. Scale bars: (I-L) 250 &mu:m, (M-P) 1μm.

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Acknowledgments

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