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Fig. 3 Mesendoderm expression of pitx2c is dependent on spt, oep, sur and cyc. In situ hybridizations using a full-length pitx2 probe are shown. (A-C) Mesendoderm expression of pitx2 at 50% epiboly. (A) Wild-type embryo. (B) spt and (C) oep mutant embryos. Animal pole views are shown with dorsal to the right. (D-I) Dorsoanterior views with the anterior at the top showing the prechordal plate expression of pitx2 at 80-90% epiboly. (D) Wild-type embryo. (E) igu, (F) spt, (G) sur, (H) cycb229 and (I) oep mutant embryos.
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