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Fig. 4 spt and papc are expressed ectopically in the midline of flh mutant embryos. Probes and embryo genotypes used are shown at the top and the bottom of the figure, respectively. The stage is indicated on the left; ep, epiboly; so, somite. The spt expression pattern in wild-type embryos is very similar to that of papc except that the anterior border of expression differs at early somite stages. In addition, in the case of papc, the anterior border is segmented. In flh mutant embryos, expression of spt and papc is not excluded from the midline, even in the somite stage. Bar, 100 μm.
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