ZFIN Image: Xu et al., 2014, Fig. 10

Image

Figure Caption

Fig. 10

Def6a and Swap70b have distinct functions in CE cell movement. In situ hybridisation with hgg1, dlx3b and ntl as indicated comparing WT embryos (A, E, I) with either Def6a (B, F, J), Swap70b (C, G, K) single morphants or Def6a/Swap70b double mutants (D, H, L). White double-headed arrows in E–H are given as reference to the WT. Arrows and vertical lines mark the hgg1 expression domain indicating the altered position and shape of the polster/prechordal plate in Def6a (B, J), Swap70b (C, K) and Def6a/Swap70b (D, L) morphants compared to WT (A, I). (M) Quantification of the altered shape of the polster/prechordal plate. The ratio of prechordal plate length to embryo diameter is shown for wild type embryos (0.19±0.02), Def6a morphants (0.31±0.06), Swap70b morphants (0.29±0.05) and double mutant morphants (0.35±0.08). Two-tailed Student′s t-tests were used to determine statistical significance. ***: P<0.001. **: P<0.01. Number of embryos (n) in each group analysed as indicated.

Figure Data

Phenotype details

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image.

Reprinted from Developmental Biology, 386(1), Xu, X., Shuen, W.H., Chen, C., Goudevenou, K., Jones, P., and Sablitzky, F., Swap70b is required for convergent and extension cell movement during zebrafish gastrulation linking Wnt11 signalling and RhoA effector function, 191-203, Copyright (2014) with permission from Elsevier. Full text @ Dev. Biol.