ZFIN Image: Xu et al., 2014, Fig. 9

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Figure Caption

Fig. 9

Ectopic GFP/Def6a expression rescued Swap70b morphants. Embryos at 2-cell stage were injected with GFP/def6a mRNA (300 pg) (A), swap70b MO (5 ng) alone (B), or GFP/def6a mRNA (300 pg) and swap70b MO (5 ng) (C) and were stained at tail-bud stage with hgg1 and dlx3b to evaluate CE using the same quantification method described above ( Fig. 4I). (D) Two-tailed Student′s t-tests were used to determine statistical significance. ***: P<0.001. (E) Embryos at 24 hpf were grouped into normal (blue), mild (green), moderate (pink) and severe (red) according to their morphological phenotype. Representative images of embryos are shown. (F) Bar graphs show the percentage of embryos with normal, mild, moderate and severe phenotypes. n: total number of embryos derived from at least three independent experiments.

Acknowledgments

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Reprinted from Developmental Biology, 386(1), Xu, X., Shuen, W.H., Chen, C., Goudevenou, K., Jones, P., and Sablitzky, F., Swap70b is required for convergent and extension cell movement during zebrafish gastrulation linking Wnt11 signalling and RhoA effector function, 191-203, Copyright (2014) with permission from Elsevier. Full text @ Dev. Biol.