Fig. 6

Fig. 6

Swap70b acts downstream of Wnt11. Zebrafish embryos at 2-cell stage were injected with GFP/swap70b mRNA (75 pg) (A), wnt11 MO (5 ng) alone (B), or co-injected with GFP/swap70b mRNA (75 pg) and wnt11 MO (5 ng) (C). (D) Quantification of A-P axis extension at 10 1/3 hpf. The angle between the most anterior and posterior embryonic structure was measured and quantified as described in Fig. 3. Two-tailed Student′s t-tests were used to determine statistical significance. ***: P<0.001. (E–M) In situ hybridisation with hgg1, dlx3b and ntl as indicated. Black double-headed arrows in H–J are given as reference to the WT. Arrows mark the hgg1 expression domain indicating the altered position and shape of the polster/prechordal plate in Wnt11 morphants (F) that is essentially rescued by ectopic expression of GFP/Swap70b (G). (N) At 24 hpf stage, embryos were grouped into normal (blue), mild (green), moderate (pink) and severe (red) according to their morphological phenotype. Representative images of embryos are shown. Each panel of the bottom two rows depict identical embryos. Green fluorescence in yolk and yolk extension is due to auto-fluorescence. (O) Bar graphs show the percentage of embryos with normal, mild, moderate and severe phenotypes. n: total number of embryos derived from at least three independent experiments.