ZFIN Image: Xu et al., 2014, Fig. 4

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Figure Caption

Fig. 4

CE cell movements are impaired in swap70b morphants. In situ hybridisation of un-injected embryos (A, C, E, G) and embryos injected with 5 ng swap70b MO1 (B, D, F, H) were performed at tail-bud stage with hgg1 (marker of the polster/prechordal plate), ntl (marker of the notochord) and dlx3b (marker of the edges of the neural plate). Medio-lateral narrowing (convergence) was quantified by measuring the ratio (I) of width of the dlx3b staining (red/black double-headed arrow) at a constant distance (1/4 of the embryo diameter) from the dlx3b arc to the embryo width (vertical black arrow). The extent of anterior–posterior lengthening (extension) was quantified by measuring the ratio (J) of the distance between hgg1 and ntl (double-headed arrow) to the embryo width (yellow scale). Two-tailed Student′s t-tests were performed to determine statistical significance. ***: P<0.001. In situ hybridisation with a myoD (G, H) revealed widening of the body axis (double-headed arrow) in Swab70b morphants.

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Acknowledgments

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Reprinted from Developmental Biology, 386(1), Xu, X., Shuen, W.H., Chen, C., Goudevenou, K., Jones, P., and Sablitzky, F., Swap70b is required for convergent and extension cell movement during zebrafish gastrulation linking Wnt11 signalling and RhoA effector function, 191-203, Copyright (2014) with permission from Elsevier. Full text @ Dev. Biol.