ZFIN Image: Recher et al., 2013, Fig. S2

Image

Figure Caption

Fig. S2

TPLSM imaging: orientation and non-invasiveness (A) Schematic drawing of a 31 hpf (prim-16) zebrafish larva head (adapted from (Kimmel et al., 1995)). Fish were mounted laterally so as to have access to the lateral side of the brain with a dipping lens objective. Red square: field of view imaged in (B). (B) 3D rendering (obtained with Mov-IT) of the imaged field at 45 hpf. Anterior part is on the left and dorsal side on the top. OT: optic tectum; Ce: cerebellum. Blue square: sagittal optical sections in (C) and (D). (C-F) Location and timing of mitoses in the PML for transgenic fish (TPLSM imaging, C-D) and WT fish (Nomarski imaging [Nikon DXM 1200 camera on Zeiss SV11 (Herbomel, 1999)], E-F). Prophase (C-E) and telophase (D-F) stages of a mitosis (arrow heads). Scale bars: 20 μm.

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Development