ZFIN Image: Venkiteswaran et al., 2013, Fig. 5

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Figure Caption

Fig. 5

Cxcr7 Modifies the Sdf1-Signaling Gradient across the Primordium at the Tissue Level

(A) Single confocal slices through mosaic primordia in live 36 hpf embryos of the indicated genotypes.

(B) Quantification of mean FmemRed/FmemGreen of host cells (black dots, gray bars SEM) and donor cells (red dots, light-red bars SEM) across the anterior-posterior axis of primordia shown in (A).

(C) FmemRed/FmemGreen ratio on the host cells only across wild-type-wild-type and cxcr7 deficient-wild-type chimeric primordia containing the Sdf1-signaling sensor. The front of the primordium is at 0 μm. Gray bars indicate SEM.

(D) Position of mosaic primordia compared to cxcr7b mutant (black rectangles) and wild-type primordia (white rectangle). The amount (heat map in μm³) and position of clonal tissue across 150 μm from the front of the schematized primordia is indicated.

Acknowledgments

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Reprinted from Cell, 155(3), Venkiteswaran, G., Lewellis, S.W., Wang, J., Reynolds, E., Nicholson, C., and Knaut, H., Generation and Dynamics of an Endogenous, Self-Generated Signaling Gradient across a Migrating Tissue, 674-687, Copyright (2013) with permission from Elsevier. Full text @ Cell