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Fig. S4
Basal processes of Muller glia collapse and markers of differentiation are downregulated after intraocular ouabain injection.
(A, B) Immunocytochemistry for the Müller glial marker glutamine synthetase (GS) (white/magenta) in unlesioned (A) and 2 dpi (B) gfap:GFP retinas with Müller glial reporter (green). GS+/GFP+ Müller glial processes in the inner retina are disrupted, whereas the organization of the outer retina, including the adherens junctions at the outer limiting membrane, remain intact at 2 dpi. (C) PCNA+ (green) activated microglia (4C4+, magenta) are abundant in the damaged inner retina at 2 dpi. (D, E) In situ hybridization for another Müller glia marker, rlbp1a (white/magenta, D), shows expression is gone at 1 dpi (E). (F) Weak Alcama immunoreactivity (magenta/ white) appears in the basal process (arrow) of a Müller glia with the inducible nGFP reporter (green) at 3 dpi. Scales: 20 μm, A-E; 10 μm, F.