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Fig. 3
Morpholino knockdown of fga, fgb, and fgg results in intracranial and intramuscular hemorrhage.
Single cell embryos were injected with 1fga, fgb, and fgg splice blocking MOs, and analyzed at 3 dpf. Larvae displayed what appeared to be hemorrhage, primarily intracranial, which was confirmed by two methods. (a) Example of an injected gata1-dsred transgenic larva with apparent hemorrhage in the facial region, confirmed under fluorescence (b). Note that the yolk sac displays autofluorescence. o-dianisidine staining detects hemoglobin, which identified sites of hemorrhage (c, d). These sites included intracranial hemorrhage with secondary hydrocephalus (arrow in d), as well as apparent intramuscular hemorrhage (arrowheads in c, d).