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Fig. S4 (A-B) The expression of the endoderm-derived pharyngeal pouches is not affected in the mutant as shown by immuno-staining with Zn5 antibody. (C-D) The characteristics of cells digested from whole embryos of WT (C) and mutant (D) genotype. Region 1 (R1) distinguishes the intact cells from debris. (E-F) The characteristics of cells digested from whole hearts of embryos with the WT (E) and mutant (F) genotype. (G-H′) A small, disorganized bulbus arteriosus (red arrow) forms at about 72 hpf in the eif3ba mutant crossed into the Tg(flk:EGFP) background (H-H′), compared with the completely-formed funneled structure in the wild-type embryo (G-G′). (G, H) Fluorescent images recorded under the confocal microscope. (G′, H′) Bright field images merged with the corresponding fluorescent images from (G, H). (I, J) Ventral view of Tg(flk:EGFP) embryos with anterior to the left, showing the abnormal OFT structure (red arrow) in the mutant compared with the normal structure in the wild-type embryo. a, atrium; v, ventricle. WT, wild type; Mut, eif3ba mutant. (G-H′) Lateral view with anterior up; (I-J) ventral view with anterior to the left.
Reprinted from Developmental Biology, 381(1), Xia, Z., Tong, X., Liang, F., Zhang, Y., Kuok, C., Zhang, Y., Liu, X., Zhu, Z., Lin, S., and Zhang, B., Eif3ba regulates cranial neural crest development by modulating p53 in zebrafish, 83-96, Copyright (2013) with permission from Elsevier. Full text @ Dev. Biol.