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Fig. 2
Expresion of otpa and otpb in wildtype larvae.
Expression of otpa and otpb were detected by double fluorescent whole mount in situ hybridization of wildtype larvae fixed at 3 dpf. From the whole confocal image stack, sub-stacks ranging from dorsal hindbrain image planes to ventral forebrain planes were used to generate a series of dorso-ventral Z-projections. The data reveal that otpa and otpb have overlapping expression but also non-overlapping domains. Dorsal view, anterior at left. Abbreviations: cH, caudal hypothalamus; HB, hindbrain; PO, preoptic region; PT, posterior tuberculum; vH, ventral hypothalamus. Scale bar is 50 μm.