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Fig. S5
Expression of hey2 is progressively downregulated with increased loss of Nkx gene dosage.
(A-E) In situ hybridization depicts expression of hey2 at 26 hpf. Lateral views, anterior to the left. hey2 expression in the ventricle (arrows) is evident in wild-type (A) and nkx2.7 mutant (B) embryos. However, hey2 expression is diminished in nkx2.5 mutant (C) and nkx2.5-/-;nkx2.7+/- mutant (D) embryos and is absent in nkx2.5-/-;nkx2.7-/- mutant embryos (E). Expression of hey2 is also visible in the diencephalon.
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