Fig. 5

Fig. 5 glypican4 mutant embryos have normal proteolytic activity. (A) Confocal images of fibronectin (Fn) expression in tailbud stage wild type and mmp14 morphant embryos. Scale bars=20 μm. (B) Fn and GAPDH western blot of 10 μg total protein extract obtained from wild type and mmp14 morphant embryos. (C) Quantification of proteolytic activity in wild type and glypican4 mutant protein extracts incubated with either gelatin or collagen IV fluorogenic substrates. Here, triplicate experiments were performed using three pools (50 embryos per pool) of total protein prepared from tailbud stage wild type or glypican4 mutant embryos.